Proteomics Core Lab.

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基本操作技術暨注意事項 | 樣品製備 | 等電點電泳儀 | 十二酯硫酸鈉-聚丙烯醯氨膠體電泳 (SDS-PAGE) | 膠體染色 | 掃描膠體並進行影像分析 | 自動取點機 | In-gel digest procedure
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|||Papers supported by Proteomics Core Lab. |||
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  • 申請貴儀中心使用費用 。長庚體系欲使用蛋白質體核心設施表列之「項目」,請編列貴儀中心使用 費用,並於網路下載「蛋白質體核心實驗室聯絡會辦表單」,送交核心實驗室加簽確認費用編列。
  • 成果發表時,請於文章之致謝欄中載明。向本實驗室申請表列之各項服務或技術支援者,請簽署「同 意書」,同意於成果發表時於文章的致謝欄 (Acknowledgment) 中載明。至於質譜分析之實驗,如膠體內蛋白質水解、質譜數據取得及分析等內容將由本實驗室提供說明 ,非蛋白質體核心設施表列之「服務項目」,以學術合作方式執行,其成果之分享等相關細節(如作者排序)則依一般學術合作慣例,由合作研究者自行擬定。原則於成果發表時,以本核心實驗室工作人員列名為共同作者。

 

 

News! (2017-9-8) Following papers supported by the Proteomics Core Lab have been recently accepted or published in journals:

  • Kung YA, Hung CT, Chien KY, Shih SR*. Control of the negative IRES trans-acting factor KHSRP by ubiquitination. Nucleic Acids Res. 2017 Jan 9;45(1):271-287. [PubMed]

  • Lin YT, Chien KY, Wu CC, Chang WY, Chu LJ, Yeh CT*, Yu JS*. (2017) Super-SILAC mix coupled with SIM/AIMS assays for targeted verification of phosphopeptides discovered in a large-scale phosphoproteome analysis of hepatocellular carcinoma. J. Proteomics.2017 Mar 22;157:40-51. doi: 10.1016/j.jprot.2017.02.005. Epub 2017 Feb 10. [PubMed]

  • Bian XL, Chen HZ, Yang PB, Li YP, Zhang FN, Zhang JY, Wang WJ, Zhao WX, Zhang S, Chen QT, Zheng Y, Sun XY, Wang XM, Chien KY, Wu Q*. (2017) Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation. Nat Commun. 2017 Feb 27;8:14420. [PubMed]

  • Lin YH, Zhen YY, Chien KY, Lee IC, Lin WC, Chen MY, Pai LM*. (2017) LIMCH1 regulates nonmuscle myosin-II activity and suppresses cell migration. Mol Biol Cell. 2017 Apr 15;28(8):1054-1065. [PubMed]

  • Chen Y-T*, Chen H-W, Wu C-F, Chu L-J, Chiang W-F, Wu C-C, Yu J-S, Tsai C-H, Liang K-H, Chang Y-S, Wu M, Ou Yang W-T. (2017) Development of a multiplexed LC-MRM/MS method for evaluation of salivary proteins as oral cancer biomarkers. Mol. Cell. Proteomics 2017 May;16(5):799-811. doi: 10.1074/mcp.M116.064758. Epub 2017 Feb 24. [PubMed]

  • Shao CH, Chen CL, Lin JY, Chen CJ, Fu SH, Chen YT, Chang YS, Yu JS, Tsui KH, Juo CG*, Wu KP*. (2017) Metabolite marker discovery for the detection of bladder cancer by comparative metabolomics. Oncotarget. 2017 Mar 21. 2017; 8:38802-38810 doi: 10.18632/oncotarget.16393. [PubMed]

  • Hsieh Y-J#, Chien K-Y#, Yang I-F, Lee I-N, Wu C-C, Huang T-Y, and Yu J-S*. (2017) Oxidation of protein-bound methionine in Photofrin-photodynamic therapy-treated human tumor cells explored by methionine-containing peptide enrichment and quantitative proteomics approach. Scientific Reports 2017 May 2;7(1):1370. doi: 10.1038/s41598-017-01409-9. [PubMed]

  • Peng Y#, Zhang M#, Zheng L#, Liang Q#, Li H#, Chen J-T, Guo H, Yoshina S, Chen Y-Z, Zhao X, Wu X, Liu B, Mitani S, Yu J-S, Ding Xue*. (2017) Cysteine protease cathepsin B mediates radiation-induced bystander effects. Nature547, 458–462 (27 July 2017) 2017 Jul 19. doi: 10.1038/nature23284.. [PubMed]

  • Cheng JC*, Tseng CP, Liao MH, Peng CY, Yu JS, Chuang PH, Huang JT, Chen JJW. (2017) Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes. Scientific Reports. 2017 Jun 30;7(1):4448.[PubMed]

  • Wu KA, Wu CC, Chen CD, Chu CM, Shih LJ, Liu YC, Wang CL, Lin HH*, Yang CY*. (2017) Proteome profiling reveals novel biomarkers to identify complicated parapneumonic effusions. Scientific Reports 2017 Jun 22;7(1):4026. [PubMed]

  • Lee SY*, Wang LJ, Chang CH, Wu CC, Chen HL, Lin SH, Chu CL, Lu T, Lu RB. (2017) Serum DHEA-S concentration correlates with clinical symptoms and neurocognitive function in patients with bipolar II disorder: A case-controlled study. Prog Neuropsychopharmacol Biol Psychiatry. 2017 Mar 6;74:31-35. [PubMed]

  • Yu CJ, Lee FJ. (2017) Multiple activities of Arl1 GTPase in the trans-Golgi network. J Cell Sci. 2017 May 15;130(10):1691-1699. [PubMed]

  • Hsiao YC#, Chu LJ#, Chen JT, Yeh TS and Yu JS*. (2017) Proteomic profiling of the cancer cell secretome: informing clinical research. Expert Review of Proteomics2017 Jul 11. doi: 10.1080/14789450.2017.1353913. [Epub ahead of print] [PubMed]

  • Hsiao Y-C#, Chi L-M#, Chien K-Y#, Chiang W-F, Chen S-F, Chuang Y-N, Lin S-Y, Wu C-C, Chu LJ, Chen Y-T, Chia S-L, Chien C-Y, Chang K-P, Chang Y-S, and Yu J-S*. (2017) Development of a multiplexed assay for oral cancer candidate biomarkers using peptide immunoaffinity enrichment and targeted mass spectrometry. Mol. Cell. Proteomics 2017 Aug 18. pii: mcp.000147.2017. doi: 10.1074/mcp.RA117.000147. [Epub ahead of print] [PubMed]

  • Chen TW#, Lee CC#, Liu H#, Wu CS#, Pickering CR#, Huang PJ, Wang J, Chang IY, Yeh YM, Chen CD, Li HP, Luo JD, Tan BC, Chan TEH, Hsueh C, Chu LJ, Chen YT, Zhang B, Yang CY, Wu CC, Hsu CW, See LC, Tang P, Yu JS, Liao WC, Chiang WF, Rodriguez H, Myers JN, Chang KP*, Chang YS*. (2017) APOBEC3A as an oral cancer prognostic biomarker in carriers of an APOBEC deletion polymorphism. Nature Commun. 2017 Sep 6;8(1):465. [PubMed]

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